Immunofluorescence staining of SIN3A in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with SIN3A Polyclonal Antibody at 4°C overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to nucleus.
Product Specifications:
Species Reactivity:
Human
Host/Isotype:
Rabbit / IgG
Class:
Polyclonal
Type:
Antibody
Immunogen:
A synthetic peptide corresponding to the N-terminus of the Human Sin3a
Conjugate:
Unconjugated
Form:
Liquid
Concentration:
1 mg/mL
Purification:
Protein A, Antigen affinity chromatography
Storage buffer:
PBS, pH 7
Contains:
0.03% Proclin 300
Storage conditions:
Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C
Target Information:
Mammalian Sin3 (mSin3) is a 150 kDa protein closely related to the yeast SIN3 repressor protein involved in the transcriptional repression of many genes. There are two mammalian isoforms, mSin3A and mSin3B. Containing 4 paired amphipathic helix domains (PAH domains), mSin3A and mSin3B have been shown to directly interact with several other transcriptional repressor proteins including HDAC1, HDAC2, RbAp46, the methyl CpG binding protein MeCP2, the Mad/Max heterodimer, and the corepressors silencing mediator of retinoic acid and thyroid hormone receptor (SMRT) and nuclear receptor corepressor (N-CoR).
