Liquid, in PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Swiss Prot:
Q16695
Gene ID:
8290
Uni-prot:
Q16695
Immunogen:
A synthetic methylated peptide corresponding to residues surrounding K4 of human histone H3
Reactivity:
Human, Mouse, Rat, Other (Wide Range)
Storage buffer:
Store at -20℃. Avoid freeze / thaw cycles.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Applications:
ChIP, IF, IHC, IP, WB
Positive samples:
HeLa acid extract, NIH/3T3 acid extract, C6 acid extract, H3 protein
Cellular location:
Chromosome, Nucleus
Background:
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.
